Cell Sorting Flow Cytometry

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Cell sorting flow cytometry.

One is by using flow cytometry and the other is by magnetically labeling the cells to differentiate and separate the cell populations. There are two methods for performing cell sorting. Cell sorting is the separation and isolation of various cell populations. Download transcript pdf slide 1.

For example if lymphocytes were concentrated to 20 million per ml the flow rate at the instrument could be run as high as 20 000 cells per second. Forward scatter fsc forward light scatter refers to the refracted light past the cell into a detector behind the laser. 12 14 31 in flow cytometers with sorting capabilities the instrument detects cells using parameters including cell size morphology and protein expression and then droplet technology. Cells larger than lymphocytes require a larger nozzle to be placed on the instrument and the flow rate is lower.

With flow cytometry cell sorting multiple detectors are being utilized to analyze different cell characteristics. If your research requires cytometric analysis our state of the art instruments acquire optical measurements using different lasers to detect fluorophores with a high level of precision. Using flow cytometry requires a flow cytometric cell sorter like the bd facsaria. Cell sorting by flow cytometry cell sorting is a method to purify cell populations based on the presence or absence of specific physical characteristics.

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