In this type of electrophoresis bacteriological acetate mem brane filters are taken in place of regular chro matography paper.
Cellulose acetate paper electrophoresis.
Electrophoresis is typically performed at ph 8 6 using cellulose acetate as the support medium.
The results were analyzed according to manufacturer s guidelines.
Cellulose acetate is the acetate ester of cellulose it was first prepared in 1865.
Cellulose acetate electrophoresis 23.
On cathode side of midline current 0 4 ma cm width of paper.
After staining strips are made transparent by soaking in whitmore s oil or liquid paraffin and then suitable for transmittance densitometry of the protein bands.
Cellulose acetate electrophoresis is an important technique in clinical diagnostics.
At this ph the overall hemoglobin molecule is negatively charged and when placed in an electric field will move towards the positive terminal anode.
It involves the following steps.
After this immerse both the ends of cellulose acetate filter paper in a buffer solution.
Here we describe a modified version of cellulose acetate membrane electrophoresis for hemoglobin separation from blood sample.
It is a modified version of paper electrophore sis developed by kohn in 1958.
Provides a good background or staining glycoprotein application.
It gives sharper bands.
Cellulose acetate electrophoresis is more or less very similar to the paper electrophoresis.
Cellulose acetate electrophoresis it contains 2 3 acetyl groups per glucose unit and its adsorption capacity is less than that of paper.
It is also used as a synthetic fiber in the manufacture of cigarette filters and playing cards in photographic film cellulose acetate replaced nitrate film in the 1950s being far.
Alternative to paper electrophoresis.
Electrophoresis on cellulose acetate paper agarose and polyacrylamide has been used as a means of separating the lipoprotein classes and indeed the separation pattern on paper electrophoresis was the basis of the fredrickson classification of lipid disorders.
The electrophoresis on cellulose acetate membrane is most widely used because of its simplicity and is without the use of any sophisticated instrument other than electrophoresis apparatus and the cellulose acetate strip.
Cellulose acetate is used as a film base in photography as a component in some coatings and as a frame material for eyeglasses.
Cellulose acetate electrophoresis the helena electrophoresis system helena laboratories beaumont tx usa was used to electrophorese hemolysates on titan iii cellulose acetate plates at 350 v for 25 min in an alkaline buffer.
The cellulose acetate system is the ideal tank for both standard and wet cellulose acetate electrophoresis.
12x2 5 cm sample applied from micropipette or capillary along a line about 1 cm.
